منابع مشابه
A rapid method of non radioactive Northern blot analysis.
The electrophoretic determination of RNA size by Northern blot analysis is an important procedure that has required to date the use of radiolabelled cDNA and cRNA probes. Here, we describe a new method which allows the size of an mRNA to be visualized by use of oligonucleotide probes directly labelled with alkaline phosphatase (AP). One advantage compared to radiolabelled probes is that AP-labe...
متن کاملSouthern and northern blot fixing by microwave oven.
Southern and Northern blot techniques are powerful procedures for studying gene structure and function. These methods involve the separation of nucleic acids according to size on an agarose or polyacrylamide gel and subsequent transfer of the nucleic acid to suitable nitrocellulose or nylon-based membranes. D N A or R N A molecules bound to the membrane are then fixed and hybridized with a radi...
متن کاملDetection of microRNAs by Northern blot analyses using LNA probes.
MicroRNAs (miRNAs) are small, endogenous, non-coding RNA species, about 21 nucleotides in length, which modulate the expression of animal and plant target genes at the post-transcriptional level. It has been shown that miRNA based gene regulation plays a pivotal role in pathways involved in growth and development. Understanding miRNA mediated processes requires new technologies enabling efficie...
متن کاملRibosomal RNA Detection on Northern Blot Employing a Specific Oligonucleotide
reconstituted basement membrane matrix is not correlated to the malignant metastatic cell phenotype. Cancer Res. 51:405-414. 4.Price, J.T. and E.W. Thompson. 1999. Models for studying cellular invasion of basement membranes. Methods Mol. Biol. 129:231-249. 5.Rodriguez, G.C., C. Haisley, J. Hurteau, T.L. Moser, R. Whitaker, R.C. J. Bast, and M.S. Stack. 2001. Regulation of invasion of epithelial...
متن کاملDistribution of L1cam mRNA in the adult mouse brain: In situ hybridization and Northern blot analyses.
Previous immunohistochemical analysis revealed a wide distribution of L1cam-positive neural and nonneural structures in adult mouse brain. Although there were numerous punctate immunoreactive nerve terminals, only a few immunoreactive neuronal cell somata were present (Munakata et al. [2003] BMC Neurosci. 4:7). To explore the distribution of L1cam mRNA-containing cells, which are interpreted to...
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ژورنال
عنوان ژورنال: Protocol Exchange
سال: 2015
ISSN: 2043-0116
DOI: 10.1038/protex.2015.080